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SKU NEL300001EA

4CN Plus substrate detects HRP and is ideal for western blotting applications. It produces dark purple precipitate in the prescence of HRP and is 10 times more sensitive than standard 4CN(4-chloro-1-napthol) formulations.

4CN Plus is a chromogenic substrate that can be used for both nucleic acid detection as well as western blotting. The amount of reagents supplied is enough to provide 750 mL of working substrate solution which is enough reagent for 3000 cm2 of membrane. 4CN Plus has been formulated for use on Polyscreen PVDF Transfer Membrane, nitrocellulose membranes and the nylon membranes Gene Screen Hybridization Transfer Membrane and Gene Screen Plus® Hybridization Transfer Membrane.

Detection method: Absorbance/Colorimetric

Components:
4CN Plus Chromogenic Substrate: 15 mls in amber bottle
4CN Plus Substrate Diluent: 75 mls of concentrated (10X) buffer in white bottle

Specifications

Specifications

Documents

SDS_NEL300001EA_EU_EN.pdf

Technical Datasheet

EN | 300.237 KB

Download

Revvity-western-blotting-solutions.pdf

Brochure

EN | 979.43 KB

Download

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