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SKU NEL937001PK

BCIP/NBT substrate detects alkaline phosphatase in blotting and slide applications. Available in ready to use solution, it deposits a permanent dark purple stain on membrane sites bearing phosphatase and with the combination of BCIP(5-bromo-4-chloro-3indolyl-phosphate and NBT(nitrobluetetrazoleum) produces much higher sensitivity than either reagent separately.

Use enough reagent to completely cover the membrane surface or at least 0.25 ml/cm2 of membrane.

This substrate solution is formulated for use on PolyScreen® PVDF Transfer Membrane, nitrocellulose membranes and the nylon membranes GeneScreen Hybridization Transfer Membrane and GeneScreen Plus® Hybridization Transfer Membrane.

Specifications

Detection MethodAbsorbance/Colorimetric
Shipping ConditionAmbient
Unit Size2x250 mL

Specifications

Specifications

Documents

SDS_NEL937001PK_EU_EN.pdf

Technical Datasheet

EN | 162.991 KB

Download

Revvity-western-blotting-solutions.pdf

Brochure

EN | 979.43 KB

Download

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