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Antifluorescein-HRP Conjugate

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SKU NEF710001EA

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Anti-fluorescein antibody conjugated to horseradish peroxidase

Antifluorescein-HRP (NEF710) and Antifluorescein-AP (NEF709) are high sensitivity detector antibodies. Both conjugates exhibit negligible non-specific binding giving them excellent performance characteristics. The reagents, sheep polyclonal antibodies conjugated to either horseradish peroxidase or alkaline phosphatase, recognize FITC and 5,6-carboxyfluorescein labeled proteins applicable to Western blots, ELlSAs and immunohistochemistry. The antibodies also recognize nucleic acids labeled with Fluorescein-N4/N6 nucleotide analogs applicable to Southern and/or Northern blots and colony/plaque screens.

When used in homologous DNA dot/slot hybridizations with a Fluorescein N6 labeled DNA probe, as little as 100 femtograms of nucleic acid are detectable with NEN Renaissance® Nucleic Acid Chemiluminescence Reagent (NEL201, NEL202) in 2 hours or NEN Renaissance® CDP Star Nucleic Acid Chemiluminescence Reagent (NEL601 ) in 5-10 minutes.

Each pack contains two 275 µL vials of antifluorescein-AP. Please refer to the certificate of analysis for working concentration information.

Specifications

Specifications
Product type

HRP Conjugates

Documents

NEF710-Technical-Datasheet.pdf

EN | 89.661 KB

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bro-western-blotting-solutions.pdf

EN | 2.692 MB

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Nova Natura UAB

Kaštonų g. 56, Giraitės k, LT-54310,
Kaunas, Lithuania

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Phone: +370 (620) 70159

Reg No 305063819

Nova Natura OÜ

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Tallinn, Estonia

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