PhenoVue™ Fluor 555 – Live Cell Tubulin Stain, is a cell permeable fluorogenic dye which specifically binds to polymerized tubulin and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Fluor 555 – Live Cell Tubulin Stain can be used to visualize microtubules in live cells, exhibiting orange fluorescence, and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 558 nm and a maximum emission wavelength of 580 nm.
PhenoVue Fluor 555 Live Cell Tubulin is a no-wash, sensitive, bright and photostable stain, which can be multiplexed with other blue, green, or far-red cell permeable stains such as PhenoVue Nuclear, Lysosomal or Mitochondrial stains.
Taxane derivatives, such as Paclitaxel and Docetaxel, which are commonly used in chemotherapy, inhibit microtubules depolymerization and cause cell cycle arrest. Like other tubulin stains derived from taxane, cytotoxicity and cell cycle impairment can be observed with long exposure time (>24h) at high concentration. This can be significantly limited at concentrations between 1 and 10 nM, while maintaining high brightness and image quality.
Depending on the cellular model, intracellular retention of PhenoVue Fluor 555 Live Cell Tubulin Stain can be further improved in the presence of efflux pump inhibitor, such as PhenoVue Probenecid, Ready to Use Solution.
Features:
- Numbers of Vials Per Unit : 1
- Quantity or Volume Per Vial: 10 nmoles
- Form: Dessicated
- Storage: -16 °C
- Recommended Working Concentration: 300 nM
- Maximum Excitation Wavelength: 558 nm
- Maximum Emission Wavelength: 580 nm
- Common Filter Set: Cy3
- Live Cell Staining: Yes
- Fixed Cell Staining: No
- Equivalent Number of Microplates:
- 6 to 20 x 96-well microplates
- 6 to 20 x 384-well microplates
- 10 to 32 x 1536-well microplates
Specifications
| Detection Method | Fluorescence |
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| Organelle or Cell Compartment | Tubulin |
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| Product Compatibility | Live samples only |
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| Product Brand Name | PhenoVue™ |
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| Shipping Condition | Dry Ice |
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| Type | Individual Reagent |
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| Unit Size | 1 |
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