PhenoVue Fluor 488 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.
PhenoVue Fluor 488 conjugated Phalloidin exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 488 - Phalloidin has a maximum excitation wavelength of 495 nm and a maximum emission wavelength of 520 nm.
Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.
Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.
PhenoVue Fluor 488 - Phalloidin can be used to vizualise actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Features
| Numbers of Vials Per Unit | 1 |
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| Quantity or Volume Per Vial | 300 units (10nmoles; 13µg) |
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| Form | Dessicated |
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| Storage | -20°C |
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| Recommended Working Concentration | 165nM (0.21µg/mL) |
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| Maximum Excitation Wavelength | 495 nm |
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| Maximum Emission Wavelength | 520 nm |
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| Common Filter Set | FITC |
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| Live Cell Staining | No |
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| Fixed Cell Staining | Yes |
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| Equivalent Number of Microplates | 2-6 x 96-well microplates
1.5-6 x 384-well microplates
3-10 x 1536-well microplates |
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For research use only.
