Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Quality
· Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing
· Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process
· Tested and found negative for bacteria, fungi, and mycoplasma
· Protein concentrations are determined by Lowry method
· Endotoxin units are measured by Limulus Amoebocyte Lysate assay
· Corning Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
· Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0mm layer of Corning Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor
Source
Engelbreth-Holm-Swarm Mouse Tumor
Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws. Do not store in frost-free freezer. KEEP FROZEN.
Application
· Cell Growth and Differentiation
· Metabolism/Toxicology Studies
· Invasion Assays
· in vitro and in vivo Angiogenesis Assays
· in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice
