Create new account

Register now and get additional benefits:
  • Personal prices
  • Delivery tracking
  • Pre-filled and quick checkout
  • Order history and fast re-ordering
  • Exact product stock status
Cart

Your cart is empty, just add some products

This is a fantastic opportunity to fill the cart with something really special!

Estimated shipping

€0.00

Total: €0.00

image/svg+xml Log in / Register
Log in or register
Log in Create account
Register now and get additional benefits:
  • Personal prices
  • Delivery tracking
  • Pre-filled and quick checkout
  • Order history and fast re-ordering
  • Exact product stock status
1-placeholder

SKU DMEM-SIL-500ML

SILAC DMEM is optimized for labeling experiments involving the use of stable amino acid isotopes in cell culture to analyze protein expression by mass spectrometry (MS).
Cat-No:DMEM-SIL-500ML
Volume:500 ml
Storage:+2°C to +8°C
Sterility: Sterile-filtere

SILAC DMEM, High Glucose (4.5 g/l), w/o L-Arginine, w/o L-Lysine, w/o L-Glutamine, w/o Phenol Red is optimized for labeling experiments involving the use of stable amino acid isotopes (SILAC = stable isotope labeling with amino acids in cell culture).

SILAC enables a simple, robust, and powerful approach in mass spectrometry (MS)-based quantitative research to explore the enormous complexity of the proteome. It is used to investigate various aspects, such as protein expression, protein quantification, and protein stability, which are difficult to detect with simple mass spectrometry.

SILAC labeling is accomplished via normal metabolic processes (e.g., cell division), by incorporating non-radioactive stable amino acid isotopes into newly synthesized proteins. In this process, the "light" amino acids contained in the growth medium are replaced by "heavy" ones. Cells growing in this medium take up the heavy amino acids and enable the differentiation between light and heavy proteins. These labeled target proteins can also be used for protein quantification. Protein levels are measured with a mass spectrometer, based on signal intensity (labeled cells appear heavier). By providing accuracy of quantification and the simplicity of interpreting MS results, the SILAC method offers unique advantages for quantitative and functional proteomics.

SILAC DMEM is formulated without L-arginine and L-lysine for multiple isotopic amino acid labeling and has no effect on cell morphology or growth rates.

Applications:

  • Quantitative and functional proteomics
  • Analyses of tissue regeneration
  • Analyses of post-translational modifications
  • MS (Mass Spectrometry)
  • NMR (Nuclear Magnetic Resonance)

A transport cost may apply to the media orders, it will be specified before shipping.

Additional information: https://www.capricorn-scientif...

Specifications

Specifications

Recently viewed

Company

Nova Natura UAB

Kaštonų g. 56, Giraitės k, LT-54310,
Kaunas, Lithuania

nova@novabio.lt
Phone: +370 (620) 70159

Reg No 305063819

Nova Natura OÜ

Valukoja 8, 11415,
Tallinn, Estonia

nova@novabio.ee
Phone: +372 5656 0050

Reg No 14758203

Let’s stay in touch

Join our newsletter!
ISO9001 1 e1593687280362