Perkin Elmer’s light-producing oncology cell lines are labeled with luciferase or dual labeled with luciferase and fluorescent reporters (GFP) for in vivo studies.
The 4T1-Red-FLuc Bioware® Brite Cell Line is a light-producing cell line derived from 4T1 mouse mammary gland adenocarcinoma. The cells have been stably transduced with the red-shifted firefly luciferase gene from Luciola Italica (Red-FLuc), for a brighter, red-shifted signal.
· Organism: Mouse
· Tissue: Mammary Gland
· Disease: Adenocarcinoma
· Optical Reporter: Red-FLuc
This cell line has very bright bioluminescence, emitting at least 15,000 photons/cell/sec in vitro. The exact number will vary depending on imaging and culturing conditions.
It’s recommended to use this line in Nude and SCID mouse models. An immune response may occur in Balb/c mice.
By emitting intensified, longer wavelength light that is significantly brighter than other firefly luciferases, Perkin Elmer’s bioluminescent oncology cell lines allow you to visualize and monitor the growth of deep tissue tumors in vivo. After the cells are injected into an appropriate mouse model, the optimized Red-FLuc luciferase enables more sensitive in vivo optical detection with less tissue attenuation so you can detect tumor development earlier, and monitor tumor growth and metastases in both subcutaneous and orthotopic models.
For laboratory use only. This product is intended for animal research only and not for use in humans.